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Vol 36(2020) N 3 p. 73-81; DOI 10.21519/0234-2758-2020-36-3-73-81
A.A. Orlova1, L.E. Aleskerova1, S.G. Vasilieva1, A.S. Morozov1, A.D. Ismailov1, E.S. Lobakova1*

Bioluminescence Toxicity Assay of Polyethylenimine-Based Sorbents

1Faculty of Biology, Lomonosov Moscow State University, Moscow, 119234 Russia

*elena.lobakova@gmail.com
Received - 21.11.2019; Accepted - 11.05.2020

References

The toxicity of polyethylenimine-based sorbents and their extracts was evaluated and their effect on the bioluminescence of Photobacterium phosphoreum photobacteria was studied. These test bacteria are commonly used as objects to determine the toxicity of various materials. The analyzed materials were synthesized by cross-linking PEI with diethylene glycol diglycidyl ether (DGDE) at mass contents of the latter of 1.9-120.0% with subsequent freezing. It was found that the degree of luminescence inhibition in the P. phosphoreum cells depended on the PEI/DGDE ratio in the sorbent. The sorbents with high DGDE content (60-120%) did not affect the cell luminescence activity, while those with a lower percentage of the cross-linker (0.9-30%) exerted a pronounced inhibitory effect on luminescence of photobacteria according to the data obtained via the standard biotesting method. It was also established that the inhibitory effect of sorbents with a lower DGDE percentage (<30%) in a phosphate buffer was significantly lower than in salt solutions. Water and ethanol extracts of sorbents with the DGDE mass percentage of more than 15% did not significantly inhibit the luminescence of P. phosphoreum during 1 h of incubation. Immobilization of P. phosphoreum cells on the surface and internal parts of the studied sorbents was observed by the method of scanning electron microscopy.

bioluminescence, biotest, toxicity analysis, photobacteria, polymer sorbents, polyethylenimine

This study was funded by the Russian Science Foundation (Grant no. 16-14-00112).



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