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Vol 36(2020) N 3 p. 46-56; DOI 10.21519/0234-2758-2020-36-3-46-56
K.A. Nikiforov1*, D.V. Utkin1, M.A. Makashova1, L.M. Kukleva1, G.A. Eroshenko1, V.V. Kutyrev1

Designing a system of multiplex PCR with hybridization-fluorescent registration of results, on solid substrate, for indication and identification Y. pestis strains

1FGHI «Russian Research Anti-Plague Institute «Microbe» of the Federal Service for Surveillance in the Sphere of Consumers Rights Protection and Human Welfare», 410005, Saratov

*Nikiforov6666666@mail.ru
Received - 22.01.2020; Accepted - 21.05.2020

References

Plague agent strains differ in intraspecific appurtenance, place of origin, as well as their virulence. Development of a rapid and reliable means of Y. pestis strain indication, their identification by intra-specific appurtenance and assessment of their virulence is a relevant issue. To solve the task, we have constructed a system of multiplex PCR with hybridization-fluorescent registration of results on solid substrate, for indication and identification of strains by their belonging to Y. pestis species, sub-species, biovars, phylogenetic lines, also by the presence of the main gene-factors of pathogenicity of chromosomal and plasmid localization. Its effectiveness is confirmed on 114 plague agent strains of various origins. The developed system of multiplex PCR with hybridization-fluorescent registration of results on solid substrate, can be applied for molecular-genetic identification of strains from natural plague foci with specification of their appurtenance to the main phylogenetic branches and assessment of virulence, for enhancement of sanitary-epidemiological control of RF territories and CIS member-states.

plague pathogen, strains, indication and identification, PCR



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