Vol 34(2018) N 4 p. 78-82; DOI 10.21519/0234-2758-2018-34-4-78-82
I.L. Glukhov*, A.A. Leont'evskij

Decolorization of Triphenylmethane Dyes by a Laccase from Bacillus pumilus

Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290 Pushchino, Moscovskaya oblast, Russia

Received - 15.05.2018; Accepted - 27.06.2018


The recombinant laccase CotA from Bacillus pumilus was evaluated for the decolorization of triphenylmethane dyes Brilliant Blue R and Brilliant Green. The process occurred within the pH range 4-10. Elevated temperatures stimulated the decolorization, the highest level being observed at 75-85 °C. Another attractive property of B. pumilis CotA was its resistance to a high concentration of sodium chloride (0.4 M NaCl at alkaline pH). The addition of the redox mediators 2,2-azino-i/5(3-ethylbenzthiazoline-6-sulfonic acid) and syringaldehyde accelerated the decolorization of the dyes by more than 3 times. The decolorization of Brilliant Green, a toxic dye effective against gram positive bacteria, resulted in its complete detoxification. The ability to decolorize under extreme conditions makes B. pumillis laccase a good candidate for dyestuff processing in industry.

laccase, Bacillus pumilus, triphenylmethane dyes, decolorization