Vol 34(2018) N 4 p. 51-61; DOI 10.21519/0234-2758-2018-34-4-51-61
D.O. Egorova*, V.A. Demakov

A strain Rhodococcus wratislaviensis КТ112-7 as a basis for Bioregeneration of PCB-Contaminated Metal/Carbon Catalyst

Institute of Ecology and Genetics of Microorganisms, Perm Federal Scientific Center, Russian Academy Sciences, Ural Branch, 614081 Perm, Russia

Received - 31.05.2018; Accepted - 06.07.2018


A possibility of the bioregeneration of polychlorobiphenyl-contaminated metal/carbon catalysts using aerobic bacteria as biological agents has been shown. Using the 5%-Pd/Sibunit catalyst contaminated by the products of the hydrodechlorinaton of the commercial mixture of PCB brand Sovol and a strain of Rhodococcus wratislaviensis КТ112-7 it was proved that the use of the bacterial culture permits to rapidly clean the catalyst with the minimal technical and economic costs. It was established that the degree of the catalyst cleaning for 24 h with the help of the intact bacteria cells was equal to 99% (0.77 mg/mL). According to the gas-liquid chromatography, the R. wratislaviensis КТ112-7 strain decomposes all the components occurring on 5%-Pd/Sibunit after the reaction of PCB hydrodechlorination. The efficiency of the bacterial destruction made up 97% (0.76 mg/mL). The residual portion of contaminating compounds was adsorbed by the bacterial cells. Therefore, the R. wratislaviensis КТ112-7 strain can be used as a biological component in the systems for PCB-contaminated metal/carbon catalyst cleaning.

Rhodococcus, catalyst, destruction, polychlorinated biphenyls

The work was performed within the framework of the state task (number of state registration 01201353246 «Search and selection of new promising microorganisms for biotechnology. The creation of immunochemical diagnostic systems».