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Vol 34(2018) N 4 p. 26-36; DOI 10.21519/0234-2758-2018-34-4-26-36
A.N. Kalinina, L.N. Borshchevskaya*, T.L. Gordeeva, S.P. Sineoky

Comparison of Xylanases of Different Origin in Expression System of Pichia pastoris: Gene Expression, Biochemical Characteristics and Biotechnological Potential

State Research Institute for Genetics and Selection of Industrial Microorganisms of National Research Center "Kurchatov Institute" (NRC "Kurchatov Institute" - GOSNIIGENETIKA), 117545, Moscow Russia

*larisa.larbor3@yandex.ru
Received - 25.05.2018; Accepted - 06.07.2018

References

The expression of the genes for endo-l,4-(3-xylanase from Bacillus pumilus VKPM A-7975, Paenibacillus polymyxa VKPM A-3015, Thermomyces lanuginosus VKPM F-224, and Schizophyllum commune VKPM F-433, members of the family of 11 glycohydrolases, in the expression system of Pichia pastoris has been assessed. Such technologically valuable characteristics of the recombinant hydrolases, as specific activity, pH and thermal stability, temperature and pH optima, resistance to digestive enzymes and xylanase protein inhibitors from cereals, and substrate specificity were investigated. It was shown that the enzyme from Paenibacillus polymyxa has the highest biotechnological potential for the creation of xylanases on the basis of the recombinant Pichia pastoris yeast strains in order to use them in the fodder production.

fodder enzymes, xylan, xylanase, Pichia pastoris.

The work was financially supported by the Ministry for Education and Science (Project Unique Identifier RFMEFI60717X0180) using Unique Scientific Organization of National Bioresource Center All-Russian Collection of Industrial Microorganisms, NRC «Kurchatov Institute»-GOSNIIGENETIKA.



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