Vol 34(2018) N 3 p. 59-66; DOI 10.21519/0234-2758-2018-34-3-59-66
N.B. Gradova*, T.V. Guseva, A.V. Malkov, and V.I. Panfilov

Environmental Assessment of Impact on Soil of Priority Pollutants of Biotechnological Productions

Mendeleev Russian University of Chemical Technology, 125480, Moscow Russia

Received - 07.03.2018; Accepted - 20.04.2018


Based on the earlier (70-ies and 80-ies) and current results, the effects on soil of microorganisms used in biotechnological processes that are indicator parameters of the emission of priority soil pollutants, have been assessed. The rate of degradation of live and inactivated biomass of Yarrowia lipolytica, Sacchammyces cerevisiae, Methylococcus capsulatus, and also soil-isolated bacteria of the Rhodococcus and Pseudomonas genera in four different soil types has been investigated. It was shown that this rate depended on type of soil and it was maximal in chernozem. It was established that the inactivated biomass and live cells of the above microorganisms in concentrations from 0.001 to 0,003 g/g soil stimulated the growth of the soil biocenosis, bacteria, fungi and actinomycetes. The soil proteolytic activity was shown to be an informative index of various levels of its contamination with the inactivated biomass. Phytotesting and growth activity of sanitary-indicative E. coli bacteria were determined as limiting parameters for biodiagnostics and bioindication of the biological contaminating factor. The concentrations of 0.002 g/g and 0.01 g/g soil were shown to be the inactive and active concentration, respectively, of the inactivated microbial biomass. The efficacy of the previously developed system of impact biological monitoring of processes connected with the production of hydrocarbon-oxidizing yeast was demonstrated in model experiments. This system takes into account the rose of wind, transfer distance of priority pollutants and uses a scheme of provoking additives within the framework of active and inactive concentrations of the biological factor to assess the effect on soil of the Rhodococcus genus bacteria and methane-oxidizing bacteria of Methylococcus capsulatus.

biotechnological production, microorganisms, inactivated microbial biomass, soil, biodiagnostics, bioindication