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Vol 34(2018) N 3 p. 33-41; DOI 10.21519/0234-2758-2018-34-3-33-41
R.I. Nuriev1,2*, L.A. Galvidis1, N.E. Yastrebova1, and M.A. Burkin1

Immunochemical and Protective Properties of Conjugated Capsular Polysaccharide of Streptococcus pneumoniae Serotype 9N

1Mechnikov Research Institute of Vaccines and Sera, 105064, Moscow Russia
2Sechenov First Moscow State Medical University, 119991, Moscow Russia

*rinatlnuriev@gmail.com
Received - 21.03.2018; Accepted - 03.04.2018

References

Conjugates of Streptococcus pneumoniae type 9N capsular polysaccharide with tetanus toxoid have been prepared. Their interactions with specific antibodies to tetanus toxoid and polysaccharide 9N were assessed, which permitted to select a conjugate with the optimal ratio of the polysaccharide and carrier protein (3:4). The parameters of the efficient adsorption of tetanus toxoid and polysaccharide on aluminum hydroxide for the following in vivo experiments were determined using the quantitative ELISA; in particular, the optimum ratio of the conjugate and adjuvant was proved be ≤ 1:1 (w/w). The repetitive immunization with the selected conjugate adsorbed on aluminum hydroxide increased the anti-PS antibody titers up to 70400 (p< 0,001) which is 140-fold higher as compared to the antigen unconjugated form. The investigation of the antibody protection from Streptococcus pneumoniae 9N after the intranasal challenge of wild-type mice revealed that the titer of the bacterial contamination in the immunized mouse lung tissue was 10-fold lower than that in non-immune mice 72 h after the contamination (p>0,05).

glycoconjugate, pneumococcal vaccine, capsular polysaccharide, Streptococcus pneumoniae.



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