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Vol 34(2018) N 3 p. 23-32; DOI 10.21519/0234-2758-2018-34-3-23-32
A.I. Kuzin1*, A.A. Tagaev2, T.V. Ovchinnikova2,3, N.I. Kuznetsova1, M.A. Nikolaenko1, O.A. Morozova3, and R.R. Azizbekyan1**

Characteristics of Bacillus pumilus B-13176 strain Producing Metabolites with Fungicide and Antibacterial Activities to Aspergillus niger and Staphylococcus aureus (MRSA)

1State Research Institute for Genetics and Selection of Industrial Microorganisms of National Research Center "Kurchatov Institute" (NRC "Kurchatov Institute" - GOSNIIGENETIKA), 117545, Moscow Russia
2Shemyakin-and-Ovchinnikov Institute for Bioorganic Chemistry, Russ. Acad. Sci., 117997 , Moscow Russia
3Sechenov First State Medical University, 119991, Moscow Russia

*aik53@rambler.ru
**raziz@genetika.ru
Received - 03.04.2018; Accepted - 22.05.2018

References

A strain B-13176 that manifested the antibacterial and fungicidal activities has been isolated by screening of sporiferous bacteria and identified as Bacillus pumilus on the basis of the analysis of variable regions of 16S RNA sequences. Cultural and morphological characteristics of the strain were studied, the dynamics of the synthesis of fungicidal and antibacterial metabolites and their location in culture were determined, and their resistance to physicochemical factors was investigated. It was shown that the strain possesses a pronounced activity against the fungi of Aspergillus niger and the methycillin-resistant bacteria of Staphylococcus aureus strain (MRSA). The culture liquid of the strain was fractioned using centrifugation, ultrafiltration, extraction and HPLC. Mass-spectrometry was used to show that the Bacillus pumilus B-13176 strain produces active thermostable protease-resistant metabolites of the peptide origin that are located in the CL pellet (the fungicidal component) and in the CL supernatant (the antibacterial component).

sporiferous bacteria, Bacillus pumilus, MRSA, producers of antibiotics, peptides.

The investigation on the isolation and characterization of the antimicrobial metabolites of the peptide origin were performed at the expense of the grant from the Russian Science Foundation (Project no. 14-50-00131).



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