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Vol 34(2018) N 2 p. 54-69; DOI 10.21519/0234-2758-2018-34-2-54-69
Yu.V. Aniskina1*, E.V. Malinovskaya2, T.V. Shalaeva1, V.S. Mitsurova1, D.A. Rodionova1, P.N. Kharchenko1, and I.A. Shilov1

Technology for Genetic Identification of Sorghum Varieties and Hybrids Based on Multiplex Microsatellite Analysis

1The All-Russian Research Institute of Agricultural Biotechnology, 127550, Moscow Russia
2The Kuban Experimental Station, Vavilov All-Russian Research Institute of Plant Genetic Resources, 352183, poselok Botanica, Gulkevichskii region, Krasnodarskii Kray Russia

*aniskina.julia@gmail.com
Received - 31.08.2017; Accepted - 01.03.2018

References

The technology for the study of genetic diversity and for genetic certification of cultured and wild sorghum plants (249 samples) has been developed on the basis of the multiplex PCR analysis of 17 polymorphic microsatellite loci in a 96-well plate followed by fluorescent detection of PCR fragments by capillary electrophoresis. The suggested approach allows to analyze each sample for a few loci simultaneously and to obtain an individual characteristic of each genotype in the collection, namely, a unique set of allele fragments the size of which is determined to the accuracy of one nucleotide. As a result, a genetic profile for each sample in the collection was obtained and a genetic passport was compiled. A significant polymorphism among the different sorghum species and varieties was found out; more than 17 alleles were identified in the majority of the loci. The established polymorphic information contents (PIC) varied in the range from 0.621 to 0.950, which indicates that the studied loci are highly informative. The dendrogram of the genetic diversity of the cultivated and wild sorghum species was constructed on the basis of the data obtained, where the majority of the sorghum samples were grouped into clusters in accordance with their classification based on morphobiological and economic features. The developed technology for the genetic identification of sorghum species allows to reliably distinguish and identify varieties, lines and hybrids as well as to control the genetic authenticity, homogeneity and hybridity of the plants. The implementation of this technology into the breeding practice will allow the genetic control of plant material at all breeding stages and the improvement of the selected forms registration and breeders' rights protection.

sorghum, genetic identification, microsatellites (SSR), multiplex PCR, DNA fragment analysis.

The work was carried out within the framework of the State Assignment "Development of New Technologies for High-Throughput Genetic Analysis of Agricultural Crops for Accelerating the Breeding Process" (Project 0574-2018-0007).



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