Vol 34(2018) N 2 p. 26-36; DOI 10.21519/0234-2758-2018-34-2-26-36
I.A. Sartbaeva1, B.N. Usenbekov2, A.B. Rysbekova2,3*, Zh.M. Mukhina4, D.T. Kazkeev5, K.Zh. Zhambakin2, E.A. Zhanbyrbaev4, Kh.A. Berkimbai2, D.Sh. Akhmetova2, and A.A. Meldebekova1

Obtaining of Doubled Haploid Lines for Selection of Glutinous Rice

1The Al-Farabi Kazakh National University, 050040, Almaty Kazakhstan
2The Institute for Plant Biology and Biotechnology (IBRR), 050040, Almaty Kazakhstan
3The Seifullin Kazakh University of Agrotechnics, 010000, Astana Kazakhstan
4The All-Russian Rice Research Institute (ARRRI), 350921, p/o Belozernyi, Krasnodar Russia
5The Kazakh National Agrarian University, 050010, Almaty Kazakhstan

Received - 01.11.2017; Accepted - 15.11.2017


A screening of zoned in the Republic of Kazakhstan rice varieties for the amylose content has been carried out. The allele state identification of the waxy gene using a marker of Glu-23 showed that all the studied varieties contain a medium amount of amylose as compared to the standard Japanese varieties. A hybridization of the Bakanasskii and Akdala Kazashstan varieties with the glutinous variety of Viola was performed to obtain low-amylose rice forms. A capacity of knotting hybrid grains in greenhouses was shown; those grains were sown in the greenhouse to obtain doubled haploids in the anthers culture. PCR was used to identify the alleles of the Wx and Alk genes in the varieties and haploid lines using the Glu-23, SNP3, SNP4, Wx_E6, Wx_E10 and dCAPS markers. Therefore, promising lines of glutinous rice with economically valuable properties to be transferred to State Trials were created using the method of routine selection and following haploid technique.

doubled haploids, glutinous variety, rice anthers culture, amylose content, waxy gene.

Acknowledgements-The authors are deeply acknowledged to the staff of the All-Russian Rice Research Institute and personally to Prof. G.L. Zelenskii, PhD Agric. Sci., for kindly provided material for selection, and theoretical and practical consulting. We are also acknowledged to Drs. Norio Iriki and T. Umemoto, employees of the Crop Breeding Research Division of the National Agricultural Research Center (NARO, Hokkaido), for the assistance in molecular genetic studies and consulting.

The work was supported by the Science Committee of the Ministry of Education and Science of the Republic of Kazakhstan within the framework of Grant Financing (Project 2168/GF 4 SC MES RK).