Vol 33(2017) N 6 p. 49-56; DOI 10.21519/0234-2758-2017-33-6-49-56
T.M. Luong1, I.A. Nechaeva1, O.N. Ponamoreva1*, H.G. Vu1, A.V. Arlyapov1, I.F. Puntus2, A.E. Filonov1,2

Effect of Low Temperature on Hexadecane Biodegradation by Oil-Degrading Bacteria Rhodoccocus sp. X5 Capable of Producing Glycolipid Biosurfactants

1The Tula State University, 300012, Tula Russia
2The Skryabin Institute for Biochemistry and Physiology of Microorganisms, 142290, Pushchino, Moskovskaya Oblast Russia

Received - 02.03.2017; Accepted - 05.07.2017


The influence of low temperature on the process of the n-hexadecane biodegradation by a Rhodococcus sp. X5 strain, a constituent of the MikroBak biopreparation, has been studied. The degrees of hexadecane degradation by the bacteria were 53% and 40% at 26 °C and 10 °C, respectively. The contents of trehalolipid biosurfactants produced by the bacteria reached 280 mg/L at 26°C and 100 mg/L at 10 °C. The cell surface hydrophobicity and total lipid content were higher at low temperature than at 26 °C: the rates of adhesion to hexadecane were 73% and 63%, and the amounts of total cellular lipids reached 169 mg/g and 115 mg/g of biomass at 10 °C and 26 °C, respectively. The correlation in the content of trehalolipid biosurfactants, cell surface hydrophobicity, content of total lipids and degree of biodegradation was established. The capacity of the physiological adaptation to low temperatures in the Rhodococcus sp. X5 bacteria determines their high potential for the bioremediation of oil-polluted territories in cold climates.

oil-degrading bacteria, biodegradation, hexadecane, glycolipid biosurfactants, low temperature, Rhodococcus.