Vol 33(2017) N 6 p. 42-48; DOI 10.21519/0234-2758-2017-33-6-42-48
L.N. Borshchevskaya*, T.L. Gordeeva, A.N. Kalinina, and S.P. Sineoky

Express Method for Identification of Industrially Valuable Yeast

The State Research Institute for Genetics and Selection of Industrial Microorganisms of National Research Centr «Kurchatov Institute» (NRC «Kurchatov Institute» - GosNIIgenetika), 117545, Moscow Russia

Received - 27.10.2017; Accepted - 01.12.2017


A method for a rapid taxonomy identification of five industrially valuable yeast groups, Pichia pastoris, Yarrowia lipolytica, Schizosaccharomyces pombe, Saccharomyces cerevisiae and Kluyveromyces lactis, has been suggested. The specific primers that were selected on the basis of nucleotide sequences of genes encoding rRNA were used in PCR. The method was effective in the identification of either pure yeast cultures or mixture of deposited yeast strain cultures. Therefore, a potential validity of this method for the identification of studied yeast in microbial preparations, at the stages of producing strains construction and during industrial fermentations was demonstrated.

yeast, identification, specific primers.

The work was performed using the financial support of the Ministry of Education and Science of RF (Project Unique Identifier RFMEFI60717X0179) and with the help of the Unique Research Set, National Bioresource Center All-Russian Collection of Industrial Microorganisms (Moscow, Russia).