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Vol 33(2017) N 6 p. 37-41; DOI 10.21519/0234-2758-2017-33-6-37-41
A.N. Kalinina*, L.N. Borshchevskaya, T.L. Gordeeva, and S.P. Sineoky

Screening and Taxonomic Characterization of Xylanase Bacterial Producers

The State Research Institute for Genetics and Selection of Industrial Microorganisms of National Research Centr «Kurchatov Institute» (NRC «Kurchatov Institute» - GosNIIgenetika), 117545, Moscow Russia

*anna.kalininaa@yandex.ru
Received - 27.10.2017; Accepted - 13.11.2017

References

A stepwise screening of bacterial producers of xylanases isolated from the forest soil in Moscow oblast, and also the taxonomic identification of the microorganisms based on the analysis of the 16S RNA gene sequences have been performed. An approach to the selection of the most active producers was suggested, and this selection was carried out resulting in 4 producing strains. Interestingly, the species to which 2 of the selected strains (Paenibacillus jamilae and P. brasilensis) belong were described as producers of xylanolytic enzymes for the first time, and the characteristics of those xylanases have not yet been studied. The new strains can serve as a source of genes for the construction of high-efficient xylanase producers.

screening, xylanases, Congo red, molecular biological identification.

The work was financially supported by the Ministry of Education and Science of RF (Project Unique Identifier RFMEFI60717X0180) and used the help of the National Bioresource Center All-Russian Collection of Industrial Microorganisms (Moscow, Russia).



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