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Vol 33(2017) N 6 p. 28-36; DOI 10.21519/0234-2758-2017-33-6-28-36
M.Yu. Shalamitskii1,2, G.I. Naumov2*

Phylogenetic Analysis of Pectinases of Ascomycetous Yeasts

1The All-Russian National Research Institute for Viticulture and Winemaking Magarach, Russian Academy of Sciences, 298600, Yalta
2The State Research Institute for Genetics and Selection of Industrial Microorganisms of National Research Centr «Kurchatov Institute» (NRC «Kurchatov Institute» - GosNIIgenetika), 117545, Moscow Russia

*naumovalb@mail.ru
Received - 28.08.2017; Accepted - 03.10.2017

References

A screening of PGU genes of ascomycetous yeasts deposited in the GenBank (http: //www.ncbi.nlm.nih. Gov/genbank/) and Sanger Institute databases (http://www.sanger.ac.uk) has been performed using the nucleotide sequence of the PGU1 gene of S. cerevisiae S288c as a query. Divergent pectinase genes were found out in the Eremothecium, Galactomyces, Geotrichum, Kluyveromyces and Lachancea genera. Within the genera, the following similarity of the PGU nucleotide sequences was observed: 64,5-982% for Kluyveromyces, 72,7-81,3% for Galactomyces/Geotrichum, and 69-87,9% for Eremothecium. Polymeric PGU genes capable of interspecies transferring were documented in Galactomyces citri-aurantii, Galactomyces candidus and Geotrichum klebahnii. The importance of the PGU genes for the diagnosis and selection of yeasts is discussed.

pectine, endopolygalacturonase, yeast pectinase, PGU gene, Eremothecium, Galactomyces, Geotrichum, Kluyveromyces, Lachancea.

The authors are acknowledged to E.S. Naumova, Chief Researcher of GosNIIgenetika, for valuable recommendations. This study was supported by the Russian Fund for Basic Research (Grant 17-04-00309).



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