Vol 33(2017) N 5 p. 61-72; DOI 10.21519/0234-2758-2017-33-5-61-72
V.Z. Krivitskaya*, E.V. Sorokin, T.R. Tsareva, M.V. Sergeeva, R.A. Kadyrova, E.A. Romanovskaya-Roman'ko, A.A. Shaldzhyan, S.V. Petrov, E.R. Petrova, N.I. Konovalova, and P.A. Petrova

Obtaining and Characteristics of Monoclonal Antibody Panel Specific to NS1 Protein of A Flu Virus

The Research Institute of Influenza, Ministry of Health of Russian Federation (NII Grippa), 197376, St.-Petersburg Russia

Received - 02.02.2017; Accepted - 12.04.2017


A panel of 7 new monoclonal antibodies that specifically interact in indirect ELISA with the fragment of the NS11-124 protein of the A/Puerto Rico/8/1934 (H1N1) virus and also with the full-size native NS1 protein in cells contaminated by A type flu viruses of various subtypes (A(H1N1), A(H1N1)pdm09, A(H2N2), A(H3N2) and A(H9N2)) has been obtained. A sensitive variant of microcultural ELISA with the use of the obtained MCA was suggested that can be helpful for the detection of the NS1 protein in infected cells and for the analysis of its antigenic variability. The results of the microcultural ELISA testify to type- and subtype-specific antigenic variability of the flu virus.

NS1 antigenic and genetic variability, NS1 protein, flu virus, microcultural ELISA, monoclonal antibodies.