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Vol 33(2017) N 5 p. 9-22; DOI 10.21519/0234-2758-2017-33-5-9-22
O.V. Karpova1,2*, A.M. Aleksandrova1, R.M. Nargilova1, and B.K. Iskakov1

In vivo Expression of Gene for AtDREB1A Transcriptional Factor under the Control of Various 5'-Untranslated Regions

1The Aitkhozhin Institute of Molecular Biology and Biochemistry, 050012, Almaty Kazakhstan
2The Institute of Plant Biology and Biotechnology, 050040, Almaty Kazakhstan

*oxkarpova@mail.ru
Received - 27.02.2017; Accepted - 12.04.2017

References

The transcription factor AtDREB1A only controls the expression of many inducible genes under low temperature conditions. The recombinant protein capable of binding with a synthetic sequence of rd29A inducible promoter from Arabidopsis thaliana has been obtained as a result of the expression of the gene AtDREB1A in E. coli. The transient expression of the gene AtDREB1A under the control of the constitutive promoter 35S CaMV and one of five different 5'-UTR (5'-TMV, -PVY, -AMV, 3xARC1 or 5'pl) was carried out in tobacco leaves simultaneously with the gene of β-glucuronidase (uidA) under the control of synthetic inducible promoter rd29A. As a result, the capacity of AtDREB1A recombinant protein to induce the in vivo transient expression of the reporter gene uidA was established. After the comparative analysis, it was shown for the first time for the AtDREB1A protein that the properties of various 5'-UTRs as translational enhancers vary greatly.

AtDREB1A, expression, inducible promoter, transgene, translational enhancer, 5'-untranslated region.

The work was performed within the framework of Research Grant Financing (4538/GF4) and Scientific-Technical Program "Development of Biotechnological Bases of Construction and Monitoring of Genetically Modified Plants with Improved Economically Valuable Properties" (О.0677) of the Ministry of Science and Education of the Kazakhstan Republic.

The authors are grateful to Dr. D.R. Gallie (University of California, Riverside, CA, USA) for the provided plasmid of pl-GUS.



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