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Vol 33(2017) N 4 p. 20-27; DOI 10.21519/0234-2758-2017-33-4-20-27
N.S. Zakharchenko*, S.V. Pigoleva, O.V. Furs, T.V. Shevchuk, O.V. Dyachenko, and Ya.I. Buryanov

Gene Expression of Antimicrobial Peptide Bombinin Increases Resistance of Tobacco Transgenic Plants to Phytopathogens

Shemyakin-and-Ovchinnikov Institute for Bioorganic Chemistry, Pushchino Branch, 142290, Pushchino, Moskovskaya oblast Russia

*znata_2004@rambler.ru
Received - 14.10.2016; Accepted - 26.12.2016

References

Transgenic tobacco (Nicotiana tabacum L.) plants with an artificial gene of an antimicrobial peptide bombinin (bom) have been obtained and investigated. The presence of the bom gene in the genome of kanamycin-resistant plants was shown by PCR. The bom gene expression was confirmed by the antimicrobial activity measurements in leaf extracts. The obtained plants were morphogenetically resistant to the Erwinia carotovora bacteria and Rhizoctonia solani fungi phytopathogens. In addition, in the transgenic plants, the protective oxidative reaction to the infection, namely the SOD activity and proline content, were lower than in the infected nontransgenic plants. The plants with the expression of the antimicrobial bombinin peptide gene are promising for the use in agricultural biotechnology as plant protectors.

antimicrobial peptides, bombinin, resistance to phytopathogens.

The work was financially supported by the Russian Fund for Basic Investigations, Grants 15-08-02050 and 16-04-00623.



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