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Vol 32(2016) N 1 p. 37-42; DOI 10.21519/0234-2758-2016-1-37-42
D. Alagoz1*, B. Binay2**, D. Yildirim3, A. Celik4, S.S. Tukel5

Improving of Stability of Formate Dehydrogenase from Candida methylica by Immobilization onto Eupergit C 250 L

1The University of Cukurova, Vocational School of Imamoglu, Adana Turkey
2The Istanbul AREL University, Faculty of Science and Letters, Department of Molecular Biology and Genetics, Tepekent, Buyukcekmece, Istanbul Turkey
3The University of Cukurova, Vocational School of Ceyhan, Adana Turkey
4The Gebze Technical University, Department of Chemistry, Gebze, Kocaeli Turkey
5The University of Cukurova, Faculty of Arts and Sciences, Department of Chemistry, 01330, Adana Turkey

*dalagoz@cu.edu.tr
**binaybaris@gmail.com
Received - 12.01.2016

References

Formate dehydrogenase (FDH) from Candida methylica has covalently been immobilized onto Eupergit C 250 L to improve its stability and reusability. The immobilization and activity yields were obtained as 80% and 42%, respectively. The optimum pH values were 7.0 and the optimum temperature values were 35°C for either free or immobilized FDH preparations. The apparent Km-kcat values were 4.18±0.22 mM-0.182±0.008 s-1 for the free FDH and 2.93±0.11 mM-0.077±0.004 s-1 for the immobilized FDH. The free FDH completely lost its initial activity at 35°C and 50 °C after 24 h of preincubation, whereas the immobilized FDH retained 84% and 77% of its initial activity under these conditions, respectively. The immobilized FDH retained 62% of its initial activity after 10 reuses in a batch type reactor.

formate dehydrogenase, Eupergit C 250 L, immobilization, stabilization



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